Studies on Browning Reaction Products from Reducing Sugars and Amino Acids

Kinetic Studies on Caramelisation of Reducing Sugars

Solubility of amino acids, sugars, and proteins*

The importance of biomolecules is well recognized nowadays, owing to their application in many industrial processes, particularly in the food, pharmaceutical, and cosmetic industries. Amino acids, carbohydrates, and proteins are the three types of biomolecules considered in this review. Solubilities of several amino acids and sugars have been measured in water and, more recently, in mixed solve...

Transport of Sugars and Amino Acids in Bacteria

Shocked cells have been examined with respect to their sensitivity to inhibitors of active transport.’ They behave similarly to unshocked cells except that their transport ability is reduced. The process of restoration of active transport has been studied. Metabolic energy appears to be necessary to restore the uptake ability of shocked cells. Evidence is presented which suggests that the galac...

Membrane transport of sugars and amino acids in isolated protoplasts.

A method has been developed for observing membrane transport in isolated protoplasts. Transport of sugars and amino acids has been studied in protoplasts isolated from the mesophyll of Pisum sativum L. That uptake was not due to passive diffusion through damaged membranes was demonstrated by supplying simultaneously two sugar stereoisomers, the one (3)H-labeled and the other (14)C-labeled. The ...

Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements.

This study evaluated the interference of the amino acids tryptophan, cysteine, histidine, tyrosine, hydroxyproline, leucine, proline, serine, glycine, valine, glutamic acid, phenylalanine, and methionine on the measurement of reducing sugars using a phenol-free 3,5-dinitrosalicylic acid (DNS) reagent. It was found that in reaction mixtures containing 20mM of either tryptophan, cysteine, histidi...